Growth of adeno-associated satellite virus in dogs in the presence of infectious canine hepatitis virus.

Pups were inoculated by varIOUS routes with adeno-associated satellite virus (ASV) of canine origin, either alone or accompanied by infectious canine hepatitis virus (ICHV). ASV was not recovered from the pups inoculated with ASV alone; however, it was recovered from one of the 5 pups inoculated simultaneously with ASV and ICHV that died on the 4th day after inoculation and showed a fever and necropsy findings typical to infectious canine hepatitis. Recovery of ASV in this pup was positive from the blood, liver, gall bladder, parotid, and bronchial lymph node; from these ICHV recovery was also positive. Although the establishment of ASV infection in dogs was not so easy, the present results suggest that ASV requires the helper virus to grow even in the natural host.